Lab Lysozyme Buffer 1, pH 8.0, 1Concentrate, 500ml/Bottle
CLP 23768
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Rigorously tested for particle content and endotoxin stability, with each batch validated to ensure quality.
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What Stands Out
Detalles de producto
- Advanced Technology: Lab lysozyme Buffer, containing 20 mM Tris, 2 mM EDTA, 1.2% Triton X-100 (W/V), pH 8.0, for the preparation and storage of lysozyme solution (this product does not contain lysozyme)
- Low Endotoxin: The packaging bottle is injection molded in one step to reduce exposure to air and reduce endotoxin
- Batch Consistency: Rigorously tested for particle content and endotoxin stability, with each batch validated to ensure quality
- Storage & Shelf Lift: Store at room temperature; Shelf life of at least 3 years
- Note: Scientific research use only; please use the reagent as soon as possible after opening to avoid affecting the results of subsequent experiments
| Brand Name | CryoKing |
| Unit Count | 1 Count |
| Manufacturer | BIOLOGIX |
| Included Components | Lab Lysozyme Buffer 1× |
| Part Number | LZ-500-1 |
| Brand | CryoKing |
| Item Weight | 37 lbs (16.78 kg) |
What's in the box
Lab Lysozyme Buffer 1×
DESCRIPCIÓN DEL PRODUCTO
Preguntas y respuestas de los clientes
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Pregunta:
What is Lab Lysozyme Buffer 1 used for?
Respuesta: Lab Lysozyme Buffer 1 is primarily used in molecular biology applications, particularly in the lysis of bacterial cells to release proteins and nucleic acids. The buffer's pH 8.0 is optimal for maintaining enzyme stability during experiments. It is frequently utilized in protein purification processes and preparation of lysates for downstream applications, such as PCR and sequencing. For example, in a laboratory setting, you can use this buffer to efficiently lyse E. coli cells for protein extraction protocols. -
Pregunta:
How should Lab Lysozyme Buffer 1 be stored?
Respuesta: To ensure the longevity and effectiveness of Lab Lysozyme Buffer 1, store it at 2-8°C when not in use. This temperature range helps maintain the stability of the buffer ingredients, preventing degradation. Always check for any signs of contamination or precipitation before use. In practice, proper storage will preserve the buffer’s integrity, making it ready for critical experiments where consistency is paramount, such as enzyme assays or diagnostic tests. -
Pregunta:
Can Lab Lysozyme Buffer 1 be used with other reagents?
Respuesta: Yes, Lab Lysozyme Buffer 1 is often compatible with a variety of reagents used in molecular biology, such as detergents, nucleases, and other buffers. However, it is essential to test compatibility depending on your specific application and experimental conditions. For instance, it can be mixed with Triton X-100 for enhanced cell lysis in protein extraction protocols. Be sure to consult specific product manuals or conduct preliminary tests to confirm compatibility before proceeding with your experiments. -
Pregunta:
Is Lab Lysozyme Buffer 1 suitable for DNA and RNA extraction?
Respuesta: Absolutely! Lab Lysozyme Buffer 1's formulation allows it to be effective in extracting both DNA and RNA from lysed cells. The buffer maintains an optimal pH that helps preserve nucleic acid integrity during the extraction process. Users often employ this buffer in conjunction with phenol-chloroform extraction or other nucleic acid purification methods for maximum yield. This makes it a reliable choice in research projects focused on cloning or genetic analysis. -
Pregunta:
How do I prepare Lab Lysozyme Buffer 1 for use?
Respuesta: Preparing Lab Lysozyme Buffer 1 for use is straightforward. Simply dilute the concentrate as per your experimental requirements, following guidelines provided in related protocols. Typically, you might want to use a 1:10 dilution, but always refer to the specific instructions for your application. Using this buffer correctly will ensure that enzymes remain active and functional in experiments, enhancing the quality of your results, such as during protein purification steps. -
Pregunta:
What is the shelf life of Lab Lysozyme Buffer 1?
Respuesta: Lab Lysozyme Buffer 1 generally has a shelf life of up to 12 months when stored properly at 2-8°C. Always check the expiry date printed on the label to ensure usability. If stored under optimal conditions, it will perform effectively in your molecular biology applications. This is particularly important for sensitive experiments that rely on the buffer's performance, such as those measuring enzyme activities or protein concentrations. -
Pregunta:
Can Lab Lysozyme Buffer 1 be used for bacterial cultures?
Respuesta: Yes, Lab Lysozyme Buffer 1 is highly effective in bacterial cultures, especially for lysis and extraction purposes. Its pH level of 8.0 helps maintain the stability of lysozyme, which is essential in breaking down bacterial cell walls. Researchers frequently leverage this buffer during experiments that involve the extraction of plasmids or recombinant proteins from E. coli strains, ensuring that the integrity and yield of their samples are maximized. -
Pregunta:
Is this buffer suitable for use in clinical laboratories?
Respuesta: Lab Lysozyme Buffer 1 is indeed suitable for application in clinical laboratories, particularly in molecular diagnostics and research involving bacteria. Its formulation supports the release and extraction of biological molecules, which is crucial when analyzing samples. Moreover, adhering to stringent protocols while using this buffer can lead to reliable results in sensitive analytical techniques, making it a valuable asset in clinical research settings focused on infectious diseases. -
Pregunta:
What are the main components of Lab Lysozyme Buffer 1?
Respuesta: The main components of Lab Lysozyme Buffer 1 typically include Tris, sodium chloride, and lysozyme which together create an optimal environment for molecular biology procedures. Tris serves as a buffering agent to maintain a stable pH, while sodium chloride enhances the overall lysing efficiency. The presence of lysozyme makes it specifically effective for degrading bacterial cell walls. Knowing the components helps researchers tailor their experiments better, especially when modifying protocols for specific bacterial strains. -
Pregunta:
Where can I buy Lab Lysozyme Buffer 1 in Chile?
Respuesta: You can buy Lab Lysozyme Buffer 1, pH 8.0, 1 Concentrate, 500ml/Bottle from Ubuy in Chile. Ubuy offers a reliable platform for sourcing high-quality lab reagents and buffers, ensuring you have the necessities for your research. Their selection often includes various laboratory supplies, making it convenient for both individual researchers and laboratory teams to find the products they need.
CryoKing Buffers Editorial Review
Customer Reviews & Ratings
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5 estrella
46%
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3 estrella
54%
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1 estrella
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ventajas
- Effective lysozyme buffer
- Stable pH value
- Good for lab experiments
- Easy to mix and use
- Reliable for various applications
Contras
- Some may find the volume excessive.
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CLP 23768
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características y beneficios
- Contains advanced lab technology for lysozyme preparation.
- Features low endotoxin levels with injection molded packaging.
- Each batch is rigorously tested for quality assurance.
- Long shelf life of at least 3 years at room temperature.
- Ideal for scientific research use.
- Ensures consistency and reliability in experiments.
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