What is Triton X-100 Lysis Buffer, and what is it used for?

Triton X-100 Lysis Buffer is a widely used solution in biochemistry and molecular biology for cell lysis and protein extraction. This 2X buffer, at a pH of 7.4, facilitates the disruption of cell membranes, allowing the release of intracellular proteins and other biomolecules. It is particularly effective for lysing mammalian cells in research applications, making it ideal for preparing samples for assays, Western blots, and other analytical techniques.

How should Triton X-100 Lysis Buffer be prepared for use?

To prepare Triton X-100 Lysis Buffer for use, you should dilute the 2X stock solution with an appropriate buffer or water, following your specific experimental requirements. It’s essential to ensure the final concentration of Triton X-100 meets your lysis needs while maintaining the desired pH of 7.4. Typically, this buffer is used in a ratio that varies between experiments, so consulting relevant protocols can help you achieve optimal results.

Can Triton X-100 Lysis Buffer be used for all cell types?

While Triton X-100 Lysis Buffer is effective for a variety of cell types, its efficacy can differ. It is particularly suited for mammalian cells due to its ability to disrupt lipid membranes. However, for particular applications or cell types (like bacterial or plant cells), using alternative lysis buffers or adjusting the concentration may yield better results. Always refer to specific protocols related to the cell line or organism you are working with for best practices.

Is Triton X-100 Lysis Buffer safe to handle?

Triton X-100 is classified as a mild detergent, which is generally safe for laboratory handling. However, it can be irritating to skin and eyes, so it's advisable to wear appropriate personal protective equipment, such as gloves and safety goggles, while working with this buffer. Following standard laboratory safety procedures and ensuring good ventilation can help mitigate any potential risks associated with its use.

What conditions should Triton X-100 Lysis Buffer be stored under?

Triton X-100 Lysis Buffer should be stored at room temperature, away from direct sunlight to maintain its stability and effectiveness. It's important to keep the buffer sealed tightly to prevent contamination. Long-term storage in a refrigerator is not recommended as it may affect the properties of the buffer. Always check the buffer before use for any changes in clarity or precipitate.

Can I freeze Triton X-100 Lysis Buffer for long-term storage?

Freezing Triton X-100 Lysis Buffer is not generally recommended, as it can lead to changes in the buffer’s properties and efficacy. The buffer is best maintained at room temperature to ensure it remains effective for its intended purpose. If longer storage is necessary, consider aliquoting smaller amounts that can be used individually, reducing exposure to light and contamination.

What volume of Triton X-100 Lysis Buffer should I use for cell lysis?

The volume of Triton X-100 Lysis Buffer needed can depend on the number of cells being lysed. Typically, a range of 100 to 300 µL of buffer per 1 million cells is used. This allows for adequate lysis while ensuring that proteins are effectively extracted. It's advisable to optimize the volume based on preliminary experiments to ensure maximum yield and efficiency for your specific cellular context.

How does Triton X-100 Lysis Buffer compare to other lysis buffers?

Triton X-100 Lysis Buffer is favored for its effective cell membrane disruption and compatibility with a wide range of downstream applications. Compared to harsher lysis buffers, Triton X-100 is milder, preserving protein functionality, making it suitable for sensitive assays. Understanding the specific needs of your experimental setup will help in choosing the right lysis buffer, whether it be Triton X-100 or another type.

What are some practical applications for using Triton X-100 Lysis Buffer?

Triton X-100 Lysis Buffer is widely used in applications such as protein extraction for Western blot analysis, enzyme activity assays, and immunoprecipitation. Its ability to solubilize proteins while maintaining their functional integrity makes it a versatile choice. Researchers often use this buffer in cell biology studies to analyze signaling pathways, investigate protein interactions, or prepare samples for mass spectrometry.

Where can I buy Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL in Chile?

You can buy Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL from Ubuy in Chile. Ubuy offers a reliable platform for acquiring laboratory supplies, ensuring you get the necessary products for your research needs. Their inventory includes various sizes and formulations, helping you to find the exact buffer you require for your experiments.

¿Cómo comprar Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL en línea desde Ubuy?

Es fácil comprar Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.

¿Está Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL disponible para comprar en línea en Chile?

Sí, en Ubuy Chile, este producto está disponible para que lo compre a un precio razonable.. El Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.

¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL

Manufactured in an ISO 9001 and 13485 certified facility in the USA, our biological buffers are essential for reliable protein research and molecular biology studies.

eCon Lab Supply

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Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL

Nº de artículo: 133308822

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What Stands Out

Dual Concentration

The 2X formulation ensures efficient cell lysis while reducing the volume needed, making experiments more effective and saving valuable reagents.

Optimal pH Level

Maintaining a pH of 7.4 is ideal for preserving protein structure and function, optimizing extraction processes in biological research.

Generous Volume

With 500mL per bottle, researchers can perform multiple experiments without frequent repurchasing, ensuring cost-efficiency and convenience in laboratory settings.

Detalles de producto

Container Type	Bottle
Item Form	Liquid
Item Weight	12 ounces (340.2 grams)
Volume	500 ml
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.118
Brand	eCon Lab Supply
Item Volume	500 ml
Package Information	Bottle

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Who Should Buy?

Biochemists
Ideal for biochemical applications requiring cell lysis and protein extraction in various experimental setups.
Cell Biologists
Great for studying cellular components by facilitating the extraction of proteins and nucleic acids from cells.
Laboratory Researchers
Useful in various research laboratories focused on molecular biology and protein assays requiring lysis buffers.

Non-Scientific Users
Not suitable for casual users or individuals without proper laboratory training or equipment for safe usage.

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Pregunta: ¿Cómo comprar Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL en línea desde Ubuy?

Respuesta: Es fácil comprar Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.
Pregunta: ¿Está Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL disponible para comprar en línea en Chile?

Respuesta: Sí, en Ubuy Chile, este producto está disponible para que lo compre a un precio razonable.. El Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.
Pregunta: ¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

Respuesta: El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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Limitaciones: Para los productos enviados al extranjero, ten en cuenta que cualquier garantía del fabricante puede no ser válida; las opciones de servicio del fabricante pueden no estar disponibles; los manuales del producto, las instrucciones y las advertencias de seguridad pueden no estar en los idiomas del país de destino; los productos (y los materiales que los acompañan) pueden no estar diseñados de acuerdo con las normas, especificaciones y requisitos de etiquetado del país de destino; y los productos pueden no ajustarse al voltaje del país de destino y a otras normas eléctricas (lo que requiere el uso de un adaptador o convertidor, si procede). El destinatario es responsable de asegurarse de que el producto puede ser importado legalmente al país de destino. Cuando hagas un pedido a Ubuy o a sus filiales, el destinatario es el importador registrado y debe cumplir todas las leyes y normativas del país de destino.
No todos los productos que aparecen en Ubuy están a la venta, ya que Ubuy es un motor de búsqueda a nivel mundial. Los productos están sujetos a las normas de exportación/comercio.

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características y beneficios

Comprehensive range of biological buffers for research and diagnostics.
Manufactured in the USA under ISO 9001 and 13485 certifications.
Critical for protein research, molecular biology, and characterization.
Produced in a certified 14644 cleanroom environment.
Triton X-100 widely used for cell lysis and membrane permeabilization.
Suitable for laboratories; not for clinical or therapeutic use.

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Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL

CLP 85023

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Detalles de producto

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Who Should Buy?

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eCon Lab Supply Buffers Editorial Review

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CLP 85023

características y beneficios

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