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Nº de artículo:
145286292
Citrate Buffer 0.2 m, pH 4.0, 1 L
Nº de artículo:
145286292
CLP 58686
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Citrate buffers can be used for RNA isolation and are a popular choice for immunofluorescent staining applications.
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What Stands Out
Consistent pH Levels
This citrate buffer maintains a stable pH of 4.0, ensuring reliability in biochemical experiments, essential for accurate results in various applications.
High Concentration
With a concentration of 0.2 M, this buffer provides effective buffering capacity, making it ideal for lab settings requiring precise conditions for enzyme reactions and other sensitive processes.
Large Volume
At 1 liter, this citrate buffer is perfect for extensive laboratory projects, reducing the need for frequent replenishment and saving time and resources in the lab environment.
Detalles de producto
| Item Weight | 2.2 lbs (1 kg) |
Who Should Buy?
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Biological Research
Ideal for experiments requiring a stable pH, such as enzymatic reactions and protein studies in biological research.
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Laboratory Use
Useful in various laboratory applications like chromatography, providing reliable results with consistent pH control.
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Sample Preservation
Effectively stabilizes samples, preventing degradation and maintaining integrity during experiments or long-term storage.
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High pH Requirements
Unsuitable for applications needing a higher pH, as its formulated pH is specifically low at 4.0.
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Certain Industrial Uses
Not recommended for large-scale industrial processes where cost-effectiveness and different pH levels are critical.
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Non-buffer Applications
Not ideal for applications that do not require a buffered environment, where other solutions may be more efficient.
DESCRIPCIÓN DEL PRODUCTO
Citrate Buffer 0.2 m, pH 4.0, 1 L
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Preguntas y respuestas de los clientes
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Pregunta:
What is Citrate Buffer 0.2 m, pH 4.0 used for?
Respuesta: Citrate Buffer 0.2 m, pH 4.0 is widely used in biological and biochemical applications, including enzyme reactions, protein purification, and maintaining the pH of samples in laboratory experiments. Its ability to stabilize pH levels makes it ideal for experiments requiring precise measurements, particularly in molecular biology techniques. For instance, in the process of electrophoresis, maintaining a stable pH is crucial to achieving accurate results. -
Pregunta:
How is Citrate Buffer prepared?
Respuesta: Citrate Buffer can be prepared by mixing citric acid and its sodium salts in a specific ratio to achieve the desired pH. For a 0.2 m concentration, carefully use a precise amount of citric acid and sodium citrate, followed by adjusting the pH with dilute sodium hydroxide or hydrochloric acid. Regular monitoring with a pH meter is essential to ensure accuracy. This preparation method is particularly useful in laboratory settings where customized buffers are essential for specific assays or reactions. -
Pregunta:
Is Citrate Buffer suitable for cell culture applications?
Respuesta: Yes, Citrate Buffer is suitable for various cell culture applications, especially when maintaining cell pH is crucial. Its phosphate-free formulation minimizes toxicity and supports the stable growth of sensitive cell lines. Researchers often use it during the preparation of culture media or during the maintenance phase to ensure optimal growth conditions, making it an essential component in cellular assays and biotechnology studies. -
Pregunta:
What is the importance of pH 4.0 in Citrate Buffer?
Respuesta: A pH of 4.0 in Citrate Buffer is significant because it falls within the optimal range for various biological reactions, particularly those involving enzymes that are active in acidic environments. This specific pH allows researchers to study enzyme kinetics, protein interactions, and other biochemical processes effectively. For example, many enzymes, such as pepsin, work best in acidic conditions, making this buffer ideal for experiments that require such environments. -
Pregunta:
Can Citrate Buffer be used in protein analysis?
Respuesta: Absolutely, Citrate Buffer is often employed in protein analysis, as it helps maintain a stable pH during the experimental procedure. Its compatibility with various proteins allows for effective analysis in techniques like chromatography and gel electrophoresis. Researchers frequently use this buffer to isolate and study proteins, as maintaining a consistent pH is crucial for preserving protein structure and function throughout the analysis. -
Pregunta:
How should Citrate Buffer be stored?
Respuesta: Citrate Buffer should be stored in a cool, dry place, ideally at room temperature and protected from direct sunlight. It is important to keep it in a tightly sealed container to prevent contamination and evaporation. Proper storage ensures its stability and effectiveness for later use, especially in critical laboratory experiments. Regular checks on the buffer’s clarity and pH are also advisable before each use to ensure optimal performance during experiments. -
Pregunta:
What are the safety precautions when handling Citrate Buffer?
Respuesta: When handling Citrate Buffer, it is essential to use personal protective equipment such as gloves and goggles to prevent skin or eye irritation. Although it's relatively safe, it's important to avoid ingestion and to work in a well-ventilated area. Always follow laboratory safety protocols, and check material safety data sheets (MSDS) for specific handling instructions. These precautions ensure safe use during various biochemical applications and experiments. -
Pregunta:
Is Citrate Buffer compatible with other lab reagents?
Respuesta: Citrate Buffer is generally compatible with a wide range of laboratory reagents, making it versatile for numerous applications. However, it's important to consider the specific chemistry of other reagents used in conjunction, as some compounds may alter the pH or stability of the buffer. Researchers often test compatibility beforehand, especially in multi-reagent experiments, ensuring that the buffer maintains its effectiveness while achieving desired outcomes. -
Pregunta:
How does the concentration of Citrate Buffer affect its function?
Respuesta: The concentration of Citrate Buffer directly influences its buffering capacity and effectiveness in maintaining pH during biochemical reactions. A 0.2 m concentration provides robust buffering ability, suitable for most biological applications. Adjusting the concentration allows researchers to tailor the buffer to specific experiments and conditions, ensuring that enzymatic reactions proceed optimally without significant pH fluctuations, which is critical for precise scientific work. -
Pregunta:
Where can I buy Citrate Buffer 0.2 m, pH 4.0, 1 L in Chile?
Respuesta: You can purchase Citrate Buffer 0.2 m, pH 4.0, 1 L from Ubuy, which offers a reliable platform for sourcing this product in Chile. Ubuy provides a user-friendly shopping experience with a variety of laboratory supplies at competitive prices.
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CLP 58686
Haz tu pedido ahora y recíbelo por ahí Sunday, Julio 05
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características y beneficios
- High-quality Citrate Buffer, manufactured in an ISO certified facility.
- Ideal for RNA isolation by preventing base hydrolysis.
- Effective in antigen detection by breaking cross-links.
- Enhances stain intensity for immunofluorescent applications.
- Meets stringent Good Manufacturing Practices and quality control.
- Perfect for biological research and clinical applications.